Review



p5e elavl3  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 92
      Buy from Supplier

    Structured Review

    Addgene inc p5e elavl3
    Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( <t>elavl3::c3</t> ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.
    P5e Elavl3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p5e elavl3/product/Addgene inc
    Average 92 stars, based on 3 article reviews
    p5e elavl3 - by Bioz Stars, 2026-05
    92/100 stars

    Images

    1) Product Images from "Pre-clinical models of idiopathic scoliosis implicate sex-specific roles for complement activity in modulating spinal curve severity"

    Article Title: Pre-clinical models of idiopathic scoliosis implicate sex-specific roles for complement activity in modulating spinal curve severity

    Journal: bioRxiv

    doi: 10.64898/2026.02.20.707049

    Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( elavl3::c3 ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.
    Figure Legend Snippet: Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( elavl3::c3 ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.

    Techniques Used: Over Expression, Clone Assay, Expressing, Transgenic Assay, Quantitative RT-PCR, Imaging



    Similar Products

    elavl3  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology elavl3
    (A) Schematic timeline of brain organoids generation from mESCs. mESCs and brain organoids developed for 7, 14 and 21 days were profiled by transcriptomics and proteomics. Part of the figure was created with Biorender. (B) Immunostaining of mESCs-derived brain organoids. Cultures of mESCs were stained with NANOG (green), POU5F1/OCT4 (red), and nuclei with DAPI (blue). Scale bars: 10 µm. (C) Organoid cryosections were stained with NESTIN and PAX6 (at day 7), <t>ELAVL3</t> and TBR1 (at day 14), and TUBB3 and GFAP (at day 21). Scale bars: 100 µm. (D) PCA showing the distribution of the transcriptomes of mESCs, brain organoids (at days 7, 14, and 21), and NBB samples. (E) Venn diagram showing the overlapping upregulated and downregulated genes between D21 organoids and NBB, both compared to mESCs. (F) Histograms of enriched GO terms in NBB vs ESCs and D21 organoids vs ESCs performed on upregulated genes (black and grey bars) and on downregulated genes (red and light red bars) determined using DAVID. For each term category (Cellular component, molecular function, and biological process), the five highest enriched terms in each gene list are shown.
    Elavl3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elavl3/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    elavl3 - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier
    anti elavl3  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc anti elavl3
    (A) Schematic timeline of brain organoids generation from mESCs. mESCs and brain organoids developed for 7, 14 and 21 days were profiled by transcriptomics and proteomics. Part of the figure was created with Biorender. (B) Immunostaining of mESCs-derived brain organoids. Cultures of mESCs were stained with NANOG (green), POU5F1/OCT4 (red), and nuclei with DAPI (blue). Scale bars: 10 µm. (C) Organoid cryosections were stained with NESTIN and PAX6 (at day 7), <t>ELAVL3</t> and TBR1 (at day 14), and TUBB3 and GFAP (at day 21). Scale bars: 100 µm. (D) PCA showing the distribution of the transcriptomes of mESCs, brain organoids (at days 7, 14, and 21), and NBB samples. (E) Venn diagram showing the overlapping upregulated and downregulated genes between D21 organoids and NBB, both compared to mESCs. (F) Histograms of enriched GO terms in NBB vs ESCs and D21 organoids vs ESCs performed on upregulated genes (black and grey bars) and on downregulated genes (red and light red bars) determined using DAVID. For each term category (Cellular component, molecular function, and biological process), the five highest enriched terms in each gene list are shown.
    Anti Elavl3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti elavl3/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    anti elavl3 - by Bioz Stars, 2026-05
    86/100 stars
    		  Buy from Supplier
    p5e elavl3  (Addgene inc)
    92
    Addgene inc p5e elavl3
    Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( <t>elavl3::c3</t> ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.
    P5e Elavl3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p5e elavl3/product/Addgene inc
    Average 92 stars, based on 1 article reviews
    p5e elavl3 - by Bioz Stars, 2026-05
    92/100 stars
    		  Buy from Supplier
    plasmid 59530  (Addgene inc)
    93
    Addgene inc plasmid 59530
    Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( <t>elavl3::c3</t> ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.
    Plasmid 59530, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid 59530/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    plasmid 59530 - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier
    paper n a recombinant dna ptol2 elavl3 h2b gcamp6s vladimirov  (Addgene inc)
    93
    Addgene inc paper n a recombinant dna ptol2 elavl3 h2b gcamp6s vladimirov
    Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( <t>elavl3::c3</t> ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.
    Paper N A Recombinant Dna Ptol2 Elavl3 H2b Gcamp6s Vladimirov, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paper n a recombinant dna ptol2 elavl3 h2b gcamp6s vladimirov/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    paper n a recombinant dna ptol2 elavl3 h2b gcamp6s vladimirov - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier
    materials availability plasmid ptol2 elavl3 h2b  (Addgene inc)
    93
    Addgene inc materials availability plasmid ptol2 elavl3 h2b
    Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( <t>elavl3::c3</t> ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.
    Materials Availability Plasmid Ptol2 Elavl3 H2b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/materials availability plasmid ptol2 elavl3 h2b/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    materials availability plasmid ptol2 elavl3 h2b - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier
    huc elavl3 antibody  (Proteintech)
    93
    Proteintech huc elavl3 antibody
    Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( <t>elavl3::c3</t> ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.
    Huc Elavl3 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/huc elavl3 antibody/product/Proteintech
    Average 93 stars, based on 1 article reviews
    huc elavl3 antibody - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier
    anti elavl3  (Proteintech)
    93
    Proteintech anti elavl3
    Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( <t>elavl3::c3</t> ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.
    Anti Elavl3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti elavl3/product/Proteintech
    Average 93 stars, based on 1 article reviews
    anti elavl3 - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Schematic timeline of brain organoids generation from mESCs. mESCs and brain organoids developed for 7, 14 and 21 days were profiled by transcriptomics and proteomics. Part of the figure was created with Biorender. (B) Immunostaining of mESCs-derived brain organoids. Cultures of mESCs were stained with NANOG (green), POU5F1/OCT4 (red), and nuclei with DAPI (blue). Scale bars: 10 µm. (C) Organoid cryosections were stained with NESTIN and PAX6 (at day 7), ELAVL3 and TBR1 (at day 14), and TUBB3 and GFAP (at day 21). Scale bars: 100 µm. (D) PCA showing the distribution of the transcriptomes of mESCs, brain organoids (at days 7, 14, and 21), and NBB samples. (E) Venn diagram showing the overlapping upregulated and downregulated genes between D21 organoids and NBB, both compared to mESCs. (F) Histograms of enriched GO terms in NBB vs ESCs and D21 organoids vs ESCs performed on upregulated genes (black and grey bars) and on downregulated genes (red and light red bars) determined using DAVID. For each term category (Cellular component, molecular function, and biological process), the five highest enriched terms in each gene list are shown.

    Journal: bioRxiv

    Article Title: The RNA and protein landscapes of mouse brain organoids

    doi: 10.64898/2026.03.13.711293

    Figure Lengend Snippet: (A) Schematic timeline of brain organoids generation from mESCs. mESCs and brain organoids developed for 7, 14 and 21 days were profiled by transcriptomics and proteomics. Part of the figure was created with Biorender. (B) Immunostaining of mESCs-derived brain organoids. Cultures of mESCs were stained with NANOG (green), POU5F1/OCT4 (red), and nuclei with DAPI (blue). Scale bars: 10 µm. (C) Organoid cryosections were stained with NESTIN and PAX6 (at day 7), ELAVL3 and TBR1 (at day 14), and TUBB3 and GFAP (at day 21). Scale bars: 100 µm. (D) PCA showing the distribution of the transcriptomes of mESCs, brain organoids (at days 7, 14, and 21), and NBB samples. (E) Venn diagram showing the overlapping upregulated and downregulated genes between D21 organoids and NBB, both compared to mESCs. (F) Histograms of enriched GO terms in NBB vs ESCs and D21 organoids vs ESCs performed on upregulated genes (black and grey bars) and on downregulated genes (red and light red bars) determined using DAVID. For each term category (Cellular component, molecular function, and biological process), the five highest enriched terms in each gene list are shown.

    Article Snippet: The primary antibodies were (species, provider; catalog number): anti-NESTIN –Rat-401- (mouse, Santa-Cruz, sc-33677); PAX6 (Rabbit, Covance, PRB-278P); NANOG (mouse, BD Pharmingen, 560259); POU5F1 (rabbit, Cell Signalling, 2840), ELAVL3 (mouse, Santa Cruz, sc-515624), TUBB3 (mouse, Covance, MMS-435P); TBR1 (rabbit, Cell Signalling, 49661), REELIN (mouse, Covance; MAB5364), GRM5 (rabbit, Millipore, AB5675), and GFAP (rabbit, Dako, Z0334).

    Techniques: Transcriptomics, Immunostaining, Derivative Assay, Staining

    Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( elavl3::c3 ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.

    Journal: bioRxiv

    Article Title: Pre-clinical models of idiopathic scoliosis implicate sex-specific roles for complement activity in modulating spinal curve severity

    doi: 10.64898/2026.02.20.707049

    Figure Lengend Snippet: Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( elavl3::c3 ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.

    Article Snippet: 2) zebrafish, p5E-elavl3 (Addgene #75025), pME-c3b.2, and p3E-polyA were recombined into pDEST Tol2 HR2 transgenesis vector.

    Techniques: Over Expression, Clone Assay, Expressing, Transgenic Assay, Quantitative RT-PCR, Imaging